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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. According to the current CDC-recommended laboratory algorithm for diagnosing HIV infection in adults and children aged >18 months, what is the recommended initial screening test?

A) HIV-1/HIV-2 antibody differentiation immunoassay
B) HIV-1 p24 antigen assay only
C) HIV-1/HIV-2 antigen/antibody combination immunoassay
D) HIV RNA nucleic acid test (NAT)

2. A clinical microbiology laboratory is investigating a cluster of bloodstream infections caused by Staphylococcus aureus. To differentiate between community-associated (CA-MRSA) and healthcare- associated (HA-MRSA) strains, they may look for the presence of:

A) Panton-Valentine leukocidin (PVL) toxin genes, which are exclusively found in HA-MRSA.
B) The mecA gene, which is common to both CA-MRSA and HA-MRSA.
C) Resistance to non-beta-lactam antibiotics, which is more common in CA-MRSA.
D) Specific staphylococcal cassette chromosome mec (SCCmec) types, such as SCCmec IV or V inCA- MRSA.

3. Staphylococcus epidermidis, a common cause of healthcare-associated infections, forms biofilms on medical devices. The production of polysaccharide intercellular adhesin (PIA) is a key factor in biofilm maturation.
The expression of PIA is primarily regulated by:

A) The icaADBC operon, whose expression is influenced by environmental cues.
B) A two-component regulatory system involving AgrA and AgrC.
C) The stringent response pathway activated during nutrient limitation.
D) Horizontal gene transfer of the ica operon.

4. A researcher is studying the genetic relatedness of different isolates of Mycobacterium tuberculosis. Which of the following molecular typing methods provides the highest discriminatory power for distinguishing between closely related strains?

A) Whole-genome sequencing (WGS)
B) Pulsed-field gel electrophoresis (PFGE)
C) Spoligotyping
D) Restriction fragment length polymorphism (RFLP) analysis

5. A patient develops a severe skin and soft tissue infection following a boating accident in the Gulf Coast.
Wound cultures grow a Gram-negative, halophilic bacterium that is oxidase-positive. The MOST likely organism is:

A) Vibrio vulnificus
B) Aeromonas hydrophila
C) Shewanella putrefaciens
D) Pseudomonas aeruginosa

Solutions: