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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Topic 2: Microbiology | 15-20% | - Specimen collection and culture techniques - Identification of pathogenic microorganisms - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology |
| Topic 3: Hematology | 20-25% | - Red blood cell physiology and disorders - White blood cell morphology and diseases - Hemostasis and coagulation testing - Routine and automated hematology procedures |
| Topic 4: Clinical Chemistry | 20-25% | - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests - Carbohydrates, lipids, proteins, and enzymes - Endocrinology and therapeutic drug monitoring |
| Topic 5: Immunology and Serology | 5-10% | - Infectious disease serology - Autoimmune and immunodeficiency disorders - Immune system principles and components - Immunologic testing methodologies |
| Topic 6: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Urine formation and composition - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing |
| Topic 7: Blood Banking (Immunohematology) | 15-20% | - Blood components and storage - Transfusion reactions and adverse events - Antibody detection and identification - Compatibility testing - Blood group systems and antigens |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Macroamylasemia can be diagnosed by measuring amylase levels in the urine and serum. In patients with macroamylasemia, there will be high levels of amylase in the serum. However, macroamylasemia can look similar to acute pancreatitis, which also causes high levels of amylase in the blood. To help differentiate, we measure amylase levels in the urine. Urine levels of amylase are low in people with macroamylasemia, but high in patients with pancreatitis.
The results which would be MOST consistent with macro-amylasemia are?
- A. Increased serum and increased urine amylase values
- B. Increased serum amylase and normal to low urine amylase values
- C. Normal serum amylase and elevated urine amylase values
- D. Normal serum and normal urine amylase values
Correct Answer: B 🗳️
Cholesterol levels do not fluctuate as triglycerides, fatty acids, lipoproteins, and chylomicrons do after an individual eats a meal. This is the reason that patients are told to fast; the laboratory needs a fasting sample to determine a lipid profile or panel which includes the latter four components.
Which of the following lipid tests is LEAST affected by the fasting status of the patient?
- A. Cholesterol
- B. Triglycerides
- C. Lipoproteins
- D. Fatty acids
- E. Chylomicrons
Correct Answer: A 🗳️
Listeria are non-capsulated, non-acid-fast, ß-hemolytic, gram-positive bacilli that have a characteristic tumbling motility. They are found primarily in the environment and in the human GI tract. Since L.
monocytogenes multiplies intracellularly, control of listeriosis requires cell-mediated immunity; thus, immunocompromised patients (such as newborns) are at high risk.
Which bacterial species is a common agent of neonatal bacteremia?
- A. Listeria monocytogenes
- B. Enterococcus faecium
- C. Streptococcus pyogenes
- D. Staphylococcus aureus
Correct Answer: A 🗳️
Gram positive organisms resist decolorization by ethyl alcohol. The large crystal violet-iodine complex is not able to penetrate the peptidoglycan layer, and is trapped within the cell in gram-positive organisms.
Conversely, the outer membrane of gram-negative organisms is degraded and the thinner peptidoglycan layer of gram-negative cells is unable to retain the crystal violet-iodine complex and the color is lost.
What is the purpose of using ethyl alcohol or acetone in the gram stain procedure:
- A. Fix all gram negative organisms
- B. Decolorize all gram positive organisms
- C. Fix all gram positive organisms
- D. Decolorize all gram negative organisms
Correct Answer: D 🗳️
Glucose positive, Maltose positive, Lactose negative and Sucrose negative is the correct answer since Neisseria meningitides causes Waterhouse-Friderichsen syndrome and it is positive only for glucose and maltose.
An autopsy of a 1-year-old female admitted to the emergency room 4 hours prior to her death revealed Waterhouse-Friderichsen syndrome. Blood and nasopharyngeal cultures taken prior to her death should reveal an oxidase-positive, Gram-negative diplococcus with the following biochemical reactions:
- A. Glucose positive, Maltose positive, ONPG positive and DNase negative
- B. Glucose positive, Maltose negative, Lactose negative and Sucrose negative
- C. Glucose positive, Maltose positive, Sucrose positive and Lactose negative
- D. Glucose positive, Maltose positive, Lactose negative and Sucrose negative
Correct Answer: D 🗳️
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